Objective To explore clinical teaching model based on maker theory in operating room nursing teaching.Methods 120 cases of clinical nursing students were randomly divided into control group and observation group, 60 in each group.The control group used conventional LBL apprentice teaching model, while the observation group introduced maker theory into the teaching practice, with more innovationsin the operating room nursing teaching. The CTDI-CV in Chinese measure, internship assessment scoresand teaching satisfaction survey of nursing students were used to evaluate teaching effects. Results The total scores and partial dimension scores in CTDI-CV scale in the observation group were significantly higher than those in the control group (P<0.05); the internship assessment scores of the observation group were significantly higher than those in the control group (P<0.05), and the students’satisfaction in the observation group was significantly higher than that in the control group (P<0.05). Conclusion The practical teaching of operating room nursing based on maker theory can effectively promote the cultivation of critical thinking and clinical comprehensive practice ability of nursing students.