Abstract: Objective To evaluate the overall quality of the computer-based test question bank for clinical medicine in a college. Methods Based on a school item bank and computer-based examination system as technical support, a computerbased test question bank was set up. From May 6 to June 24, 2017, 2432 people participated in the test, and one-way analysis of variance (ANOVA) was used. The reliability (R), difficulty (P), and degree of differentiation (D) were used as indicators for evaluation. Results Except for endocrinology, nephrology and orthopedics, the test question bank of the other 14 departments were all stable (P-value ＜ 0.05); except for the fourth exam of neurosurgery, the difficulty D was 0.85 and the second examination of orthopedics has a reliability R of 0.56, the difficulty, reliability, and differentiation of the questions in other departments are within the reference range (0.51-0.84, 0.62-1, 0.13-1). Conclusion The reliability of the examination questions in each department of the university’s computer-based test questions database is relatively high, and the difficulty is moderate. It can better separate students’knowledge mastery and can be used to promote other medical schools.