Abstract: Wang Tinghuai’s teaching team of Zhongshan School of Medicine of Sun Yat-sen University has been carrying out the reform of teaching mode of physiology since the early 1980s. Experimental physiology teaching mode was gradually changed from single, closed and verified to multi-disciplinary, open and exploratory. It gained better teaching effect and cultivated the innovative awareness, the scientific thinking and researching ability of medical students. Experimental physiological science became the National Essential Course in 2009. This paper briefly reviewed and explored the reform process of experimental physiology teaching of Wang Tinghuai’s teaching team for reference.