Abstract: Objective To study the measures and effectiveness of nursing work in disinfection supply room after the application of prospective management concept. Methods The disinfection supply room in our hospital adopted the prospective management from September 2014 to September 2015,and applied relevant management concept into the nursing work, mainly include integration of information, human resources, the popularity of PDCA circulation management method and 5S management method, etc. At the same time, an investigation were carried out among 15 staff to check their performance in cleaning, washing, disinfecting and packing medical materials. Results Before the prospective nursing management, qualified rate of cleaning, disinfecting, packing and distributing accounted for 90.00%, 85.50%, 82.50% and 87.50% respectively; in comparison, after the prospective nursing management, qualified rate of these four items accounted for 98.50%, 100.00%, 99.00% and 100.00% respectively. The differences between before and after the application of prospective management were significant(P <0.05). Conclusion The nursing management in disinfection supply room should learn from prospective management concept, staff should work in line with scientific, rigorous and standardized health care service requirement and ensure each medical material of hospital in high security.