Abstract: Objective To discuss about the method and the effect of the grouping-management on the special nurses in the operating room of the special hospital. Methods We executed the grouping-management on the special nurses in our operating room during the period of the study. Through this way, we wanted to analyze the differences between the foreand- aft grouping-management in some aspects including the prepare of the instruments for operating, the instruments transmission, the mastery of the doctors’ habits, the degree of the cooperation between the doctors and the nurses, the mastery of the knowledge and the skills of the nurses, and so on. Results After executing the grouping-management, for those nurses, the degree of the proficiency of the instruments transmission, the mastery of the doctors’ habits, the cooperation between nurses and doctors, the mastery of the knowledge and skills were better than before this grouping-management (P <0.05).Conclusion There existed the obvious effect in the clinical practice after executing the grouping-management in the operating room in the special hospital. Through this way, we could improve the levels of the proficiency of the instruments transmitting and preparing, the mastery of the doctors’ habits, the cooperation between the doctors and nurses, what’s more, we could also enhance the mastery of the knowledge and the skills for the nurses. In a word, this management should be promoted to be used in the clinical practice.