Abstract: Objective To explore the teaching effect of the micro teaching platform of laboratory diagnostics. Methods Clinical students in Class 2014 of Kunming Medical University were selected as experimental subjects, and divided into experimental group and control group. In the teaching of laboratory diagnostics, traditional classroom teaching methods were used in the control group, while traditional classroom teaching combined with the micro teaching were applied to the experimental group in order to compare teaching effects of two teaching mothods in the final exam and teaching satisfaction. Results After the completion of teaching work, the final score of the experimental group was significantly higher than that of the control group, the difference was statistically significant (P<0.05). After the end of the course, student satisfaction in experimental group with micro teaching was significantly higher than that in the control group in terms of the teaching methods and teaching effect, the difference was statistically significant (P<0.05). Conclusion The teaching method combined with the micro teaching can significantly improve the students' learning effect of laboratory diagnostics and arouse the students' enthusiasm for learning.