Abstract: Objective To Learn the health status of the hospital's medical staff, and explore health factors, so as to develop targeted measures to improve the physical and mental health of the staff. Method In December 2015, we selected a top three hospital in Tai'an, Shandong Province as the investigation site and used general survey method to investigate the hospital's medical staff. A questionnaire in four parts, including basic information, health status, labor intensity and lifestyle, and job satisfaction, was sent to them. A total of 1105 people who’s questionnaire was qualified were included in our study. Results Health status: The prevalence rate of chronic diseases of the medical staff was 24.3%. The population of male over 51 years old with advanced title and engaged in this profession for more than 30 years have the higher prevalence. The top three diseases with the highest prevalence were chronic gastroenteritis, hypertension and cardiovascular disease. Health factors: 49.5% of the respondents had high workload; 52.4% of the respondents had high pressure; 70.1% of the respondents worked more than 8 hours per day; 62.5% of the people had daily meals irregularly; and 69.8% of the people hardly participated in physical exercise. Job satisfaction: 15.0% of the medical staff were not satisfied with the current job. Binary logistic regression analysis demonstrated that predictors of medical staff’s job satisfaction were age, marital status, educational background and job type (P<0.05). Conclusion The respondents’ health status were poor and it is urgent to ease their work pressure. During the management work in the future, the hospital should always be concerned about the physical and mental health of the medical personnel and take various measures to alleviate their workload and pressure, and improve their job satisfaction.