Abstract: Objective To evaluate the implementation outcomes of the “Maternal and Child Health” Micro-major jointly offered by the School of Public Health and the affiliated Women and Children’s Hospital of Chongqing Medical University, in cultivating interdisciplinary maternal and child health talents. Methods A mixed-method approach combining qualitative and quantitative methods was used to conduct a pre- and post-course comparative assessment among the inaugural cohort of 23 third-year preventive medicine undergraduates, covering core knowledge tests, a self-directed learning competence scale, helpfulness evaluations of teaching components, and surveys on course utility and satisfaction. Results The completion rate reached 100%. Core knowledge mastery rate rose from 71% pre-course to 96% post-course (χ²=4.17, P=0.04). Among self-directed learning ability dimensions, interpersonal communication showed significant improvement (t = 2.13, P = 0.041). Forty-eight percent of students rated the course as exceeding their expectations, while 35% reported meeting their expectations. “Experiential Kitchen” (5.0 points), “Clinical Case Analysis” (4.9 points), and “Hospital Site Visits” (4.6 points) scored highest in stimulating learning interest and enhancing application ability, whereas “Lecture-based Instruction” (2.6 points) in practical sessions scored the lowest. Conclusion Through the construction of an interdisciplinary curriculum and practice platforms, this Micro-major has effectively improved students’ knowledge and competencies. However, optimization of course density and practical teaching models still need further optimization.