Abstract: Objective To evaluate the reliability and validity of the Course Experience Questionnaire (CEQ) for evaluating class teaching quality in a medical university and to describe the distribution characteristics of CEQ scores provide scientific basis for improving the teaching quality of medical education. Methods 329 university students from different grades and majors who have completed four courses were selected by adopting the random cluster sampling method to conduct the questionnaire survey.The properties of CEQ including reliability(internal consistency reliability and split-half reliability), structural validity (exploratory factor analysis) and content validity (correlation between the total score of CEQ and the scores of common factors) were assessed.The SPSS 17.0 was adopted for data description and analysis. Results A total of 313 students returned responsive questionnaires.The Cronbach’s α coefficient of CEQ is 0.962, and the Spearman-Brown coefficient is 0.861. In the structural validity evaluation, totaled 3 common factors were extracted from exploratory factor analysis, explaining 77.895% of the total variance. There was a positive correlation between the total score of CEQ and class quality and harvest factor, as well as good teaching factor, and there was a positive correlation between class quality and harvest factor and good teaching factor (P ＜ 0.001). The total score of CEQ questionnaire for 313 medical students was 4.044±0.752, the score of class quality and harvest factor was 4.149±0.790, the score of good teaching factor was 4.282±0.632, and the score of appropriate workload factor was 2.153±0.633. There were statistical significant differences in the CEQ scores of the four courses (P ＜ 0.05), and the CEQ score of the early contact practice training courses which accounted for 44.4% of practice classes, was the highest. Conclusion The application of CEQ in the assessment of medical students’ course experience demonstrates good reliability and validity. Students hold a positive attitude to course experience,which is above average.