Abstract: Objective To explore the application effect of the teaching mode of flipped Classroom combined with microlecture in the teaching of basic theory of pediatrics. Methods A total of 64 medical students of the five-year Grade 2014 were selected from clinical medicine of Dalian Medical University. Experimental group was teached by flipped classroom combined with microlecture teaching method; control group traditional teaching method. Each group have 32 students. After the completion of the teaching, the teaching results are evaluated through theoretical examinations and questionnaires. The questionnaire survey includes learning interests, learning effects, and students’ satisfaction with the teaching model.Results The scores of the skill operation test in the control group were (79.09±5.80) lower than those in the experimental group (82.78±3.00), and the two groups were statistically different (P<0.05).The test scores of the control group were (81.09±5.49) lower than that of the experimental group (87,47±3.78), and the two groups were statistically different (P<0.05). In the control group, 20 people were interested in skill operation, and 29 people in the experimental group. The difference between the two groups was significant (P<0.05).18 people were satisfied with the model of teaching in the control group, and 30people were satisfied in the experimental group. The difference between the two groups was significant (P<0.05). Conclusion Flipped classroom combined with microlecture teaching method has a good teaching effect and is worth promoting. This method can cultivate students’ analytical ability and clinical thinking ability, and can also improve students’ interest in pediatrics, so as to achieve better learning results and get classmates’ satisfaction.