Abstract: Objective To investigate the difference between the cognition of medical staff and AIDS infected people in the current situation of humanistic care, and find out the problems and resistance of humanistic care in the process of diagnosis and treatment. Methods A total of 752 medical staff and 460 AIDS infected persons were investigated by questionnaires to analyze the cognition, attitude and admissions on AIDS patients and AIDS knowledge mastery of medical staff. And the current status of humanistic care was analyzed. Results 89.36% medical staff and 90% AIDS infected people consider that humanistic care can promote the treatment effect; 31.12% of medical staff did not participate in the training of humanistic care; The correct rate of medical staff on the mastery of AIDS transmission routes was 93.83%; 36.3% infected people had the experience of being rejected from hospital. 56.12% medical staff and 36.30% infected people think that AIDS infected people trust medical staff. 92.29% medical staff and 85.87% infected people support team mode of humanistic care for AIDS infected people. Conclusion In the process of medical services for AIDS infected people, medical staff may reject to provide medical service because of discrimination and occupational exposure. The degree of humanistic care for AIDS infected people is generally low. It is essential to strengthen the training of moral education, AIDS knowledge,prevention of occupational exposure, humanistic care knowledge and doctor-patient communication skills. Team mode of humanistic care for AIDS infected people should be supported.