Abstract: Objective To explore the application value of case-based teaching(CBL)combined with evidence-based medicine thinking in clinical laboratory immunology experimental teaching. Methods Clinical laboratory students of Grade 2016 in class A were assigned to the control group, used traditional teaching method, while students in class B were assigned to the experimental group, and used CBL integrated with evidence-based thinking teaching method as well as traditional teaching method. The examination scores and questionnaires were used to evaluate the teaching effectiveness.Results More than 90% students are in favor of the teaching method of CBL integrated with evidence-based thinking. It helps students to understand and memorize experimental knowledge, and improve their clinical performance. The average examination scores in the experimental group was 84.5±5.1 and those in the control group was 88.1±4.3: the scores of clinical case analysis (Total score of 40) in the experimental group was 27.2±3.7 and the control group was 36.3±4.1.Conclusion CBL integrated with evidence-based thinking teaching method can enhance students’ ability of analyzing and solving problems when they meet clinical cases, and help students have better command of clinical laboratory immunology examination techniques.