Abstract: Objective To investigate the teaching effect of the evidence-based medicine(EBM) course among medical students of Beijing Tongren Hospital affiliated to Capital Medical University and explore the improving methods. Methods A total of 65 medical students,including thirty-one 5-year students and thirty-four 7-year students, were enrolled. Their examination paper was analyzed and the frequency of complete accuracy of each knowledge point was calculated. The comparison of the 5-year students and the 7-year students was made by Chi-square test. Opinions and suggestions were collected among the 5-year students. Results　There were nine knowledge points with the frequency of complete accuracy exceeding 50% among 5-year students while there were only four among 7-year students and the other seven points were below 50%. All the students mastered the concept of EBM, but the perceptions towards randomized control trial were not good. The 5-year students were better than the 7-year students in mastering the two knowledge points: the concept of systemic review (80.65% vs 41.18%, P=0.001) and the level of evidence (61.29% vs 32.35%, P=0.019). Some 5-year students suggested that more chances of participation in the course should be given to them and hoped to go on learning EBM in their clinical practice. Conclusion　The analysis of the above results may improve the teaching methods and strategies of the EBM course for the 5-year and 7-year medical students. It will benefit the teaching of the EBM course in the clinical practice stage.