Abstract: Objective To evaluate the application of seminar teaching method in standardized training of general surgery residents. Methods 35 students received standardized training in General Surgery Department of Xuanwu Hospital,Capital Medical University from September 2016 to August 2018 were selected and divided into the experimental group and the control group by random number table method, with 35 students in each group. Seminar teaching method and the traditional teaching method were used in the experimental group and the control group respectively. Results Structured clinical examination (OSCE) and student satisfaction survey were used to evaluate teaching efficacy. Results Scores of the medical history collection and physical examination, case analysis, basic operation skills and auxiliary examination results in the OSCE examination of the experimental group were higher than those of the control group (P ＜ 0.01). The scores of rationality in the curriculum design, the study enthusiasm, ability of clinical thinking and research have been improved and were higher than those of the control group (P ＜ 0.01). Conclusion Seminar teaching method is helpful to improve the quality of standardized training for general surgery residents.