Abstract: Objective To investigate the knowledge of organ donation among ICU medical staff and their attitudes towards organ donation after death. Methods The questionnaires designed by ourselves were applied in the survey on ICU medical staff, and 106 valid questionnaires were obtained from 34 doctors and 72 nurses separately. The contents of questionnaire include general information, knowledge of organ donation and attitudes toward organ donation. Results The average score of ICU medical staff’s knowledge about organ donation was (7.30±1.45), which was in the middle level. The score was related to identity, educational background and professional title. 61.3% approve of organ donation after death,show no difference between doctors and nurses. Conclusion In order to further procure the potential donors, it’s necessary to popularize the knowledge about organ donation, strengthen publicity to promote the work of organ donation.