Abstract: Clinical probation is an essential process of medical education and an important means of training clinical thinking and learning clinical skills. Evidence-based medicine (EBM) is a new model of medical research, and also a method of clinical learning. The introduction of EBM into clinical probation teaching is of great significance to improve the teaching content and methods of medical colleges and to enrich the clinical practice of probationers. Through the experiment of evidence-based medicine teaching mode for clinical interns in a top-level hospital in Beijing, this study found that the teaching effectiveness of evidence-based medicine teaching mode in clinical internship was remarkable, and it was helpful to cultivate the comprehensive ability of clinical medical students and to arouse the interest of clinical medical students in clinical work and medical research.