Abstract: Objective To fully understand the present situation of teaching staff in medical university and college affiliated hospitals in Yunnan province. Method The author worked out a questionnaire to investigate a total of 56 hospitals affiliated to the universities and colleges across the province. Questions covered the number of teachers and talent distribution, structure of educational background and job titles and the teaching consciousness. Results Most medical university and college-affiliated hospitals in Yunnan province have established a relatively complete management system of the teaching staff and were able to support clinical teaching in accordance with the standards. Among the affiliated hospitals,those of first class at grade 3 had the absolute advantage in number, educational background and job titles of the teaching staff compared with hospitals at grade 3 of second class and hospitals at grade 2. Meanwhile, such problems as non-balanced distribution, unreasonable structure, uneven teaching excellence and inadequate support and fund investment from relevant university or college to the hospital were found in the clinical teaching staff in affiliated hospitals. Conclusion Colleges and universities should establish a clinical faculty development center; build a faculty team of high medical ethics and good skills and a dynamic adjustment mechanism, develop and further improve the incentive mechanism of clinical teaching;design teachers’ training courses oriented by their clinical post competence; build a number of teaching teams consisting of both fundamental and clinical teachers to adapt to the reform of the organ system-based curriculum.