Abstract: Objective To study the effect of step-by-step training mode in standardized training of residents in ultrasound specialty, and to provide basis for standardized training of residents. Methods The residents who completed the training and examination in Department of Vascular Ultrasound, Xuanwu Hospital from 2011 to 2017 were continuously included. In 2011-2014 years, the traditional “imparting” teaching method was used as the control group. In the 2015 －2017 years, the “step-by-step” training mode was used as the experimental group. The pass rate of theoretical test and skill test of the two groups were compared in the first stage of residents in Beijing. Result In totality, 28 residents were included in the current study, namely, 14 in the control group and 16 in the experimental group (2 of them did not passed the 2014 examination). The pass rate of the theoretical assessment was 100% and 93.75% in the experimental group and the control group, respectively (χ2=1.096, P=0.485); the pass rate of the skill assessment was 94.12% and 47.37%, respectively. The step-by-step training mode improved the pass rate of skill assessment significantly, and there was a significant difference (χ2=12.389, P<0.001). Conclusion The effect of the step-by-step training mode is obviously better than that of the traditional training, and it can be further applied in the training of resident doctors in ultrasonic specialty.