Abstract: Objective To analyze the current status of staff in tertiary hospital taking paid leave and the factors contributing to the status, and to provide policy recommendations for the further development of public hospital personnel system reform. Methods　Samples were drawn by using multi-stage stratified random sampling and 450 questionnaires were completed, data were collected and analyzed by using logistic regression. Results The percentage of staff having taken paid leave in the past year was 90.5%,about 57% of them had exhausted all their paid leave. General management in department, employee position and types of employment contract were the influencing factors for the staff. The difference between departments (χ2=8.979,p<0.05), employee position (χ2=86.187,p<0.001) and type of employment contract (χ2=29.974, p<0.001) were statistically significant, respectively. Conclusion　Medical staff work load is great, and they have much less in-depth and comprehensive understanding of the paid vacation system. Different types of employees are all likely to be at higher risk, management concept is the key to implement the system of paid vacation. Tertiary hospital personnel system reform should increase awareness of non-economic salary system, and build an employee centered culture to meet the future challenges of doctor's multi-sited license.